Rousseau C, Vaidya S, Hall A, Creanor S, Girkin J,Whitters CJ (2002) The effect of underlying dentine on QLF measurements of artificial caries.. Caries Res. 36: p. 190
	This study aimed to determine the contribution of underlying dentine to the fluorescence scattering measurements of artificial caries recorded using quantitative light fluorescence (QLF). Sixteen 5- mm2 blocks were cut from the labial surfaces of extracted bovine incisor teeth stored previously in thymol. Tissue was removed using a dental drill from the dentine side of half of the block to just beyond the enamel-dentine junction. The blocks were mounted in epoxy resin for ease of handling and the enamel surface polished with an Al2O3 slurry. Half of the enamel surface at right angles to the axis of dentine removal was covered with acid-resistant varnish and the exposed enamel placed in a lactic-acid containing demineralising solution (Carbopol) for 72 h. Mineral loss was determined by comparing the fluorescence scattering of sound enamel and demineralised enamel on areas of the block with no underlying dentine (site A) and then with underlying dentine (site B). Data were available for 15 specimens. A paired t-test demonstrated no difference between the two sites (p = 0.43) and a correlation of 0.97 was noted for the two data sets. Furthermore, fluorescence intensity measurements across the sound and demineralised areas of the specimen demonstrated no effect from underlying dentine. In this study it was apparent that underlying dentine had no effect on the fluorescence scattering measurements made by QLF.

 

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