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A fiber-optic fluorescence observation (FOFO) technique has been developed for the quantification of demineralized root dentin. The method was tested on 40 specimens of in vitro demineralized parts of human root dentin. Fluorescein sodium salt was used as a penetrating dye. The fluorescein sodium salt was excited using light around 465 nm. The fluorescence signal around 527 nm measured for demineralized dentin was corrected for lamp output and then divided by the corrected fluorescence signal for the sound control dentin on the same root, resulting in a FOFO-value. FOFO-values correlate linearly (r = 0.91) with mineral loss measured by transverse microradiography. Therefore, it was concluded that the FOFO-technique provides a good, non-destructive measure of the severeness of in vitro demineralized human root dentin, and is applicable on bulk dentin. Future work on natural lesions in vivo will be needed to investigate the applicability of the technique in a clinical situation. |